Product: Hy-Fy High Fidelity Mix (x2)

Hy-Fy High Fidelity Mix (x2) is a premixed, ready-to-use solution containing High Fidelity DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. High Fidelity Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding intensifier and optimizer.

High Fidelity DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable High Fidelity DNA polymerase possesses 3′ to 5′ exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that High Fidelity DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using High Fidelity DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. High Fidelity DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Comes in 1ml / 5x1ml